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Step-by-step procedure

Authors: Emma Tubæk Nielsen, MD., Caroline Lilja, MD., and Magnus Balslev Avnstorp, MD.
Illustrator: Emma Tubæk Nielsen, MD.

This chapter explains and illustrates two different types of gracilis flap:

  1. Gracilis myocutaneous free flap.
  2. Gracilis free muscle transfer.

Gracilis myocutaneous free flap

  1. Pre-operatively using a doppler, the vascular supply is identified and marked. The pedicle is usually identified 5-10 cm inferiorly to the pubic tubercle.
  2. The patient is placed in a supine position. The patient’s leg is placed abducted, slightly externally rotated, and flexed.
  3. The surgical field – reaching from the knee to the inguen – is prepped and draped.
  4. A line going straight from the pubic tubercle to the medial femoral condyle is drawn, marking the axis of the gracilis muscle – approximately two to three finger widths posterior to the adductor longus.
  5. Flap size – equivalent to the defect at the recipient site – is marked as an elliptical skin flap, centered on the gracilis muscle axis. The flap can be placed according to orientation need, being placed transversely, obliquely, or longitudinal to the gracilis muscle.
  6. Distally, the tendon of the gracilis tendon is palpated just proximally to the medial femoral condyle, and the incision site is marked.
  7. The doppler is used to confirm the location of vascular supply. The arteria is marked on the skin.
    • The nerve innervation of the gracilis muscle is usually located about 1,5 cm superior to the vascular pedicle.
  8. Local anesthesia is injected along the edges of the flap.
  9. A proximal incision is made through the skin, moving distally along the anterior line of the elliptical draft.
  10. Dissection of the superficial saphenous vein is performed, and the vein is preserved.
  11. Dissection through subcutaneous tissue and down to the fascia of the gracilis muscle.
  12. The fascia is elevated off the muscle anteriorly, and the septal junction between the gracilis and adductor longus muscles is identified.
  13. The adductor longus is retracted to identify the vascular pedicle (the medial circumflex femoral artery). Vessel loops are placed to ensure correct identification of the artery and vein.
  14. An incision is made corresponding to the marking at the gracilis tendon insertion site. A vessel loop is placed around the tendon.
  15. Dissection is continued toward the medial circumflex femoral artery, allowing for additional vessel length. It is important to ensure the vascular pedicle is as long as possible. The pedicle is dissected distally to proximally, ligating branches to the adductor longus and magnus until the pedicle is reached.
    • If preserved motor function is indicated, the nerve is identified superior to the vascular pedicle and harvested with the flap. The nerve should be dissected as far up as possible, usually at the bifurcation of the obturator nerve.
  16. Dissection is extended around the gracilis muscle to isolate the muscle.
  17. The posterior border of the elliptical draft is incised and dissected is continued through the subcutaneous tissues.
  18. The muscle belly is incised inferiorly at the desired flap length.
  19. From distal to proximal end, the flap is elevated by freeing the gracilis muscle until it is only attached to the vascular pedicle, the nerve and its superior/proximal insertion point.
  20. Perforating vessels extending from the gracilis muscle to the skin island are preserved. Vessel clips are placed to ensure optimal vessel handling.
  21. At the desired length, the muscle is incised superiorly/proximally.
  22. The vascular pedicle is clamped medially and divided together with the nerve.
  23. Hemostasis is secured before direct closing the donor site in 3 layers. Closure is preferably done with sutures in the fascia, dermal inverters sutures and intradermal suture but can also be done with a simple continuous suture.
  24. Before completing the closure, a wound drain is placed distally to the incision and secured with a drain knot followed by either a half hitch or double half hitch. The knot should be close to the skin surface.
  25. Compression bandages are applied to minimize swelling, bleeding and seroma.1, 3, 7
Illustration of upper arm muscle anatomy
1

Step 1: Preoperative positioning and marking

  • Pre-operatively using a doppler, the vascular supply is identified and marked. The pedicle is usually identified 5-10 cm inferiorly to the pubic tubercle.
  • The patient is placed in a supine position. The patient’s leg is placed abducted, slightly externally rotated, and flexed.
  • The surgical field – reaching from the knee to the inguen – is prepped and draped.
  • A line going straight from the pubic tubercle to the medial femoral condyle is drawn, marking the axis of the gracilis muscle – approximately two to three finger widths posterior to the adductor longus.
Diagram of dissected human arm showing muscles
2

Step 2: Flap size and placement

  • Flap size – equivalent to the defect at the recipient site – is marked as an elliptical skin flap, centered on the gracilis muscle axis. The flap can be placed according to orientation need, being placed transversely, obliquely, or longitudinal to the gracilis muscle.
Illustrated skin incision diagram with scalpel.
3

Step 3: Proximal skin incision

  • A proximal incision is made through the skin, moving distally along the anterior line of the elliptical draft.
  • Dissection of the superficial saphenous vein is performed, and the vein is preserved.
  • Dissection through subcutaneous tissue and down to the fascia of the gracilis muscle.
Surgical incision with scalpel and retractors on arm.
4

Step 4: Identification of the vascular pedicle.

  • The fascia is elevated off the muscle anteriorly, and the septal junction between the gracilis and adductor longus muscles is identified.
  • The adductor longus is retracted to identify the vascular pedicle (the medial circumflex femoral artery). Vessel loops are placed to ensure correct identification of the artery and vein.
  • An incision is made corresponding to the marking at the gracilis tendon insertion site.
Surgical wound showing exposed subcutaneous tissue.
5

Step 5: Gracilis tendon identification

6

Step 6: Identification of vascular pedicle and obturator nerve

  • Dissection is continued toward the medial circumflex femoral artery, allowing for additional vessel length. It is important to ensure the vascular pedicle is as long as possible. The pedicle is dissected distally to proximally, ligating branches to the adductor longus and magnus until the pedicle is reached.
  • If preserved motor function is indicated, the nerve is identified superior to the vascular pedicle and harvested with the flap. The nerve should be dissected as far up as possible, usually at the bifurcation of the obturator nerve.
  • Dissection is extended around the gracilis muscle to isolate the muscle.
  • The posterior border of the elliptical draft is incised and dissected is continued through the subcutaneous tissues.
Leg dissection showing muscles and tendons.
7

Step 7: Flap elevation

  • From distal to proximal end, the flap is elevated by freeing the gracilis muscle until it is only attached to the vascular pedicle, the nerve and its superior/proximal insertion point.
Forearm surgical procedure illustration.
8

Step 8: Dissection and harvest of vascular pedicle and obturator nerve

  • Perforating vessels extending from the gracilis muscle to the skin island are preserved. Vessel clips are placed to ensure optimal vessel handling.
  • At the desired length, the muscle is incised superiorly/proximally.
  • The vascular pedicle is clamped medially and divided together with the nerve.
Arm with surgical stitches and drainage tube.
9

Step 9: Donor site closure

  • Hemostasis is secured before direct closing the donor site in 3 layers. Closure is preferably done with sutures in the fascia, dermal inverters sutures and intradermal suture but can also be done with a simple continuous suture.
  • Before completing the closure, a wound drain is placed distally to the incision and secured with a drain knot followed by either a half hitch or double half hitch. The knot should be close to the skin surface.
  • Compression bandages are applied to minimize swelling, bleeding and seroma

Gracilis free muscle transfer

  1. Pre-operatively using a doppler, the vascular supply is identified and marked. The pedicle is usually identified 5-10 cm inferiorly to the pubic tubercle.
  2. The patient is placed in a supine position. The patient’s leg is placed abducted, slightly externally rotated, and flexed.
  3. The surgical field – reaching from the knee to the inguen – is prepped and draped.
  4. A line going straight from the pubic tubercle to the medial femoral condyle is drawn, marking the axis of the gracilis muscle – approximately two to three finger widths posterior to the adductor longus.
  5. Flap size – equivalent to the defect at the recipient site – is marked as an elliptical skin flap, centered on the gracilis muscle axis. The flap can be placed according to orientation need, being placed transversely, obliquely, or longitudinal to the gracilis muscle.
  6. Distally, the tendon of the gracilis tendon is palpated just proximally to the medial femoral condyle, and the incision site is marked.
  7. The doppler is used to confirm the location of vascular supply. The arteria is marked on the skin.
    • The nerve innervation of the gracilis muscle is usually located about 1,5 cm superior to the vascular pedicle.
  8. Local anesthesia is injected along the edges of the flap.
  9. A proximal incision is made through the skin, moving distally along the anterior line of the elliptical draft.
  10. Dissection of the superficial saphenous vein is performed, and the vein is preserved.
  11. Dissection through subcutaneous tissue and down to the fascia of the gracilis muscle.
  12. The fascia is elevated off the muscle anteriorly, and the septal junction between the gracilis and adductor longus muscles is identified.
  13. The adductor longus is retracted to identify the vascular pedicle (the medial circumflex femoral artery). Dissection is continued toward the medial circumflex femoral artery, allowing for additional vessel length. It is important to ensure the vascular pedicle is as long as possible. The pedicle is dissected distally to proximally, ligating branches to the adductor longus and magnus until the pedicle is reached.
  14. If preserved motor function is indicated, the nerve is identified superior to the vascular pedicle and harvested with the flap. The nerve should be dissected as far up as possible, usually at the bifurcation of the obturator nerve.
  15. The dissection is extended around the gracilis muscle to isolate the muscle.
  16. The posterior border of the elliptical draft is incised and dissected is continued through the subcutaneous tissues.
  17. The muscle belly is incised inferiorly at the desired flap length.
  18. From distal to proximal end, the flap is elevated by freeing the gracilis muscle until it is only attached to the vascular pedicle, the nerve and its superior/proximal insertion point.
  19. Perforating vessels extending from the gracilis muscle to the skin island are preserved. Vessel clips are placed to ensure optimal vessel handling.
  20. At the desired length, the muscle is incised superiorly/proximally.
  21. The vascular pedicle is clamped medially and divided together with the nerve.
  22. Hemostasis is secured before direct closing the donor site in 3 layers. Closure is preferably done with sutures in the fascia, dermal inverters sutures and intradermal suture but can also be done with a simple continuous suture.1, 3, 7
  23. Before completing the closure, a wound drain is placed distally to the incision and secured with a drain knot followed by either a half hitch or double half hitch. The knot should be close to the skin surface.
Illustration of upper arm muscle anatomy
1

Step 1: Preoperative positioning and marking

  • Pre-operatively using a doppler, the vascular supply is identified and marked. The pedicle is usually identified 5-10 cm inferiorly to the pubic tubercle.
  • The patient is placed in a supine position. The patient’s leg is placed abducted, slightly externally rotated, and flexed.
  • The surgical field – reaching from the knee to the inguen – is prepped and draped.
  • A line going straight from the pubic tubercle to the medial femoral condyle is drawn, marking the axis of the gracilis muscle – approximately two to three finger widths posterior to the adductor longus.
Illustrated arm with dashed line diagonally across
2

Step 2: Incision marking

  • The incision site is marked.
Scalpel making incision on skin during surgery.
3

Step 3: Proximal incision

  • The doppler is used to confirm the location of vascular supply.
  • The nerve innervation of the gracilis muscle is usually located about 1,5 cm superior to the vascular pedicle.
  • Local anesthesia is injected along the incision site.
  • An incision is made going from most proximal and moving distally along the incision site.
Illustrated infected surgical wound on skin
4

Step 4: Dissection of the superficial saphenous vein, and elevation of fascia

  • Dissection of the superficial saphenous vein is performed, and the vein is preserved.
  • Dissection through subcutaneous tissue onto the fascia of the gracilis muscle. The fascia is elevated from the muscle anteriorly, and the septal junction between the gracilis and adductor longus muscles is identified.
Anatomy of thigh showing adductor longus muscle.
5

Step 5: Dissection of the vascular pedicle

  • Dissection is continued toward the medial circumflex femoral artery, allowing for additional vessel length. It is important to ensure the vascular pedicle is as long as possible. The pedicle is dissected distally to proximally, ligating branches to the adductor longus and magnus until the pedicle is reached.
  • If preserved motor function is indicated, the anterior branch of the obturator nerve is identified superior to the vascular pedicle and harvested with the flap. The nerve should be dissected as far up as possible, usually at the bifurcation of the obturator nerve.
Illustration of forearm incision surgery procedure
6

Step 6: Muscle harvest preparation

  • The gracilis is harvested according to recipient site needs. Generally, the anterior 50% muscle width (or whole muscle width) and about 10 cm of muscle is harvested.
  • The point of muscle harvest is centered over the pedicle and the nerve, at the point of muscle entry.
Surgical arm anatomy illustration with veins and nerves.
7

Step 7: Gracilis muscle harvest

  • The muscle segment is detached from the remaining muscle. 
Arm fasciotomy illustration with surgical incision details.
8

Step 8: Dissection and harvest of vascular pedicle and obturator nerve

  • The vascular pedicle is clamped proximally and divided together with the nerve.
Surgical procedure with exposed wiring and incision
9

Step 9: Flap transfer to recipient site

  • The muscle flap is now prepared for transfer to the recipient site.

References

  1. Kim, Jason H. Gracilis tissue transfer. Medscape, Clinical procedures. 24.07.2019. Link:https://emedicine.medscape.com/article/880792-overview#a2
  2. Fattah A. Y. et al. A three-dimensional study of the musculotendinous and neurovascular architecture of the gracilis muscle: application to functional muscle transfer. Journal of Plastic, Reconstructiv & Aesthetic Surgery, 2013.  
  3. Universitat Autònoma de Barcelona. Dissection in Fresh Cadaver. European Master’s degree in surgical oncology, reconstructive and aesthetic breast surgery (MRBS).
  4. Buntic, Rudy. The Gracilis Flap. Microsurgeon, Flaps. Link: https://www.microsurgeon.org/gracilismuscle
  5. Dr Zezo. Gracilis flap. Plastic Surgery Key. 03.03.2017. Link: https://plasticsurgerykey.com/gracilis-flap/
  6. Franco M. J. et al. Lower Extremity Reconstruction with Free Gracilis Flaps. J Reconstr Microsurg 2017.
  7. Redette R. J. et al. Limb salvage of lower-extremity wounds using free gracilis muscle reconstruction. Plastic and Reconstructive Surgery 2000.
  8. Wax M. K. et Azzi J. Perioperative considerations in free flap surgery: A review of pressors and anticoagulation. Oral Oncology 2018.

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